Thursday, December 26, 2019

Outsourcing Outsourcing And Outsourcing - 1840 Words

Outsourcing, and in particular offshore outsourcing, is absolutely necessary and helps our country s economy. Outsourcing helps a company focus on those things it does best and hence increase its top line revenues while reducing costs. Outsourcing has provided organic growth to the United States corporations and enabled them to compete more effectively in global markets. This paper seeks to explore what outsourcing is, what the difference between outsourcing and offshoring is, what effects it has on individuals, firms, countries and the world as a whole, the controversy surrounding outsourcing, trends of outsourcing and the necessity for it. Outsourcing refers to sending any type of work that a business would otherwise be completed†¦show more content†¦For example, Wyeth Pharmaceuticals outsourced its clinical-testing operation to Accenture Ltd. in 2004 (Engardio, Arndt, Foust, 2006). Even the local city and state government also outsources services like infrastructure to build roads, toll booths, and health care, trash and snow removal. Outsourcing can free up government agencies to focus on strategy, planning, and core functions they perform best and governing the people. Outsourcing is more prevalent than you might know, and it is very vital to our economy. Offshoring or offshore outsourcing is the relocation of a business process from one country to another. For example, a business in the US could outsource work to a contractor within the US which is outsourcing. If the business instead outsources the work to a foreign contractor like in India or the Philippines, it is called â€Å"Offshoring†. While outsourcing is used to access contractors with advanced skills locally for short term projects or to speed-up the projects for completion, offshoring is used basically to reduce the cost than in-house staff for a task or a process. Firms offshore anything that could be from simple customer care to more critical operations like research and development (RD), total information technology (IT) management, and new product development. Even though these may seem like core competencies, some firms still outsource them, so

Tuesday, December 17, 2019

Causes And Effects Of Social Media - 1874 Words

Causes and Effects of Social Media There is no denying that the expansions of the World Wide Web and social media have skyrocketed in the last few years. It all started with the birth of MySpace for Generation Z in 2003. It then happened again later in 2004, when Mark Zuckerburg created the most commonly known and used Facebook, which then led to the launch of Twitter in 2006. Ultimately, what all of this is saying, is that the effects of social media have impacted the lives of many since 2003. All of these social sites piggyback off of one another and it has created a nation that has become dependent on the use of social media. People are using social media as a way to get their news, instead of actually watching the news. Instead of†¦show more content†¦This birth was known as Twitter. Twitter was launched in 2006. The purpose of Twitter was to create a â€Å"mini blogging† site, hence the reason that its users are limited to 140 characters per tweet. In 2009, it wa s ranked as the top 3 social sites out of the ones mentioned and multiple more. As time progressed, Instagram was released in 2010. Instagram is similar to Facebook because one is able to share photographs, but that is Instagram’s sole purpose. Instagram allows the users to upload photos, edit those photos, and write captions to go along with them. Moving along, in 2011, a different form of social media came out. The famous Snapchat that quickly escalated. Snapchat allows one to send and receive snapchats that dissolve after 10 seconds or less. It began where one could only send pictures and receive them, but then the creators attended the instant messaging part. The messages, much like the pictures, are gone as soon as one is finished it with it. It is easy to see how quickly social media progressed and in a very small time frame. The impact social media has made is very evident and it has created a never ending list of possibilities because of this drastic expansion. Since social media surfaced, it has created many new opportunities, whether it be for businesses or just keeping up to date with what one’s favorite celebrity is doing. With the advancement of socialShow MoreRelatedCauses And Effects Of Social Media1049 Words   |  5 Pageswhat effect social media has on people worldwide. Many people are using social media daily and because of that, they might not know the effects that can be lurking behind a screen and causing harm to their mental health. Social media has many negative effects on mental health. Some people could think that mental health and social media has no relation, when it does. It is a fast-growing issue with many â€Å"cause and effect† reasons behind it. Each cause needs to be recognized along with the effect itRead MoreCauses And Effects Of Social Media Essay769 Words   |  4 Pagesthe past years, social media has grown tremendously fast. Most people have at least one social media account. The use of social media has its benefits and its problems. It is good for spreading the current news, advertising businesses, and it is one of the easiest and fastest way people communicate with each other. However, it also spreads hate. Some people let their social media rule their life as if they must live up to something because of what they witness on the social media. The popularityRead MoreCause and Effect of Social Media Essay888 Words   |  4 Pagesyounger generation in particular. Technology plays as a key factor in most lives, social media especially. Among the most popular of social media are Facebook, Twitter and Instagram, all with the main purpose to share or view personal information and experiences, communicate with others and meet new people on social or professional grounds. There are several positives and negatives effects associated with social media, making it a topic of debate whether it is creating more good or harm for today’sRead MoreSocial Media Negative Effects O n People1255 Words   |  6 Pages Social media negative effects on people psychology paper Research Question: How Social media has negative effects on people? Review of literature: Social Media may seem positive and safe, but they affect our daily lives more than you can think of. If someone is sad or mad it could be because they have just used social media . It’s true people’s emotions can change because of social media. Due to this fact I got curious andRead MoreNegative Effects Of Social Media Essay1209 Words   |  5 PagesStatistics show that teens spend nearly 9 hours a day on social media websites, which is more than one-third of their day (Tsukayama). Social media, among teenagers and young adults, is quite popular. It is used on many different platforms and in so many ways which allows high schoolers to be constantly attached. Over the past decade, with the emergence of social media, the amount of time these websites consume from individuals has become greater and greater. Parents who have grown up in a differentRead MoreSocial Media And Its Effects On The Individual1665 Words   |  7 Pages The average teenager spends more time on social media than sleep. Explain the causes of the increase in social media use, and the effects on the individual. Are the effects largely positive or negative? This essay will begin by explaining defining what social media is and when it came into existence, following this, research will be used to identify what factors have contributed to the exponential rise in social media use globally as well as referring to available statistics on howRead MoreNegative Effects Of Social Media1297 Words   |  6 PagesIn the twenty-first century, social media plays an extensive part in most people’s lives. Instagram, Twitter, and many other social networks can cause adverse psychological harm and even lead to physical harm. Although many may argue that social media pertains to be useful, there prove to be more harmful aspects rather than helpful aspects of these media sites. These effects are not only psychological, but they may also be physical. Not only do social networks cause depression, anxiety, and cyberbullyingRead MoreThe Effects Of Social Media On Children s Development1067 Words   |  5 PagesAbstract As children are exposed to more and more social media with the increase of technology, it is important that parents minimize the exposure of social media to their children. There are multiple extensive ways in which social media can influence a child’s well-being and development: An influence on their social ability with others, the content of the social media, and the activities that social media replaces such as exercise. Methods that could potentially improve the risk of a child’s well-beingRead MoreThe Effects Of Social Media On Teenagers1379 Words   |  6 PagesThe Social Media Effect When you were a teenager, did social media impact your life? If not, it certainly has had an impact on the teenagers of America today. Social media has been around for a long time. There are many applications for social media, some of which are Twitter, MySpace, and Facebook. With the rapid evolution of social media, people believe that social media is impacting teenagers in a negative way. However, some believe that there are positive effects. The negative effects ofRead MoreNegative Effects Of Social Media1350 Words   |  6 Pagestoday’s society, social media is an important tool that many people use daily for various purposes. On social media, people share their opinions, events in their lives, and news. They also use social media as a tool for entertainment and as a way of keeping in touch with their friends. Because of its various uses, the use of social media increased very significantly over the years and it is no surprise that many scholars from different disciplines took an interest in the use of social media. These scholars

Monday, December 9, 2019

Accounting for Managers Accounting Policies and Solutions

Question: Describe about the Accounting for Managers for Accounting Policies and Solutions. Answer: Introduction This study deals with analyzing four major problems in relation with management accounting. In this particular assignment, emphasis has been given on management accounting forming the core part of every business organization (Libby, Rennekamp and Seybert 2015). This particular business organization aims at taking several decisions based on results of management accounting. It gives detailed solutions to the four problems listed in the assignment and highlighting the major importance. Problem 1 Part A This question requires describing the differences in accounting policies that majorly affects financial statements of company for specified time. Company A uses straight-line depreciation method. On the other hand, Company B follows double declining method. There is huge difference in the process and it has wide impact on the given financial statements of business enterprise (Kaplan and Atkinson 2015). These figures will be reflecting in the book value of assets. It is known that Straight-Line methods make use of residual valuation of asset. As far as double declining method is concerned, it is calculated based on (1/useful life*200). Calculation has been provided in the Appendix 1 of the assignment. It depicts the depreciation calculation of Company A and Company B. Cost of assets is estimated at 100,000 and salvage value is 10000. There is a difference of book valuation presented in the middle years for certain asset. For instance, if a particular company sells its asset for 80000 for six year time period, then Company A will record profit at 7000 (80000-73000). This has to be shown in the profit and loss statement. On the contrary, Company B profit will be recorded at 26856 (80000-53144). On analysis, it has been noticed that Company B has more profit generation in comparison with Company A (Horngren et al. 2013). Addition to that, Company A assets will be declining because of the book value as it reflects upon higher valuation in comparison with Company B. Hence, it can be understood that this type of difference present in the depreciation treatment widely affects financial statements of Company A and Company B for specified period. Part B This particular question asks for the information regarding depreciation disclosures as mentioned in different types of financial statements (Healy and Palepu 2012). It requires proper understanding of adequate knowledge that has been transmitted to the reader by gaining depreciation information presented in the financial statements. Therefore, it is noted that depreciation treatment is based upon three financial statements such as cash flow statement, balance sheet as well as income statement. These three statements reveal the fact from business perspectives. As far as balance sheet is concerned, depreciation reflects upon total amount of accumulated depreciation as incurred by the fixed assets for ant business organization. It is therefore understood that accumulated depreciation is done for specified time. It is the amount of accumulated depreciation that is to be credited when a particular journal entry is passed (Hartley 2014). For Instance, in case life of fixed assets is treated for 10 years as mentioned in the financial statements for preparing it for 3 years. In that case, accumulated depreciation will infer for total first years of double balancing method. As far as cash flow statement is concerned, it deals with total amount of depreciation that is added back to the net income for various non-cash transactions (Guo-liu and Sui 2013). In case of income statement, depreciation is shown as an expense as well as posses negative impact especially on net income. Addition to that, depreciation in the income statement considers as the amount that is debited at the time of passing the journal entry for a particular transactions. The above-mentioned information is regarding the knowledge depicted by a reader for depreciation of financial statements for future analysis purpose (Drury 2013). Problem 2 This particular problem is concerned with the medium-sized company named as Kangaroo Express. It requires description of three alternative types of financing for the company for Kangaroo Express. It further needs explanation on the risk as well as return implications for each of the alternatives for Marsupials (Droms and Wright 2015). In this case, Kangaroo Express can easily opt for various sources of financing for bringing improving in the capital structure. These types of sources of funds usually come from equity financing as well as debt financing. Equity Financing Debt Financing Venture capitalists Bank Loans Initial public offerings, Asset backed mortgage loans Retained earnings Government Bonds Table: Different types of equity as well as debt financing (Source: Dhamija 2015) The above table points out the various types of equity financing and debt financing in accordance with the medium-sized enterprise named as Kangaroo Express Venture Capitalists Venture capitalists are one type of equity financing that provides capital to business enterprise. It is in exchange with share ownership for given business organization. In case Kangaroo Express opts for venture capitalists, cost of return will be huge in nature (Collier 2015). Therefore, there is huge risk present in this case, as it will dilute the ownership. Kangaroo Express has shares of Marsupial family, and then it will be huge trouble for them in the near future. Initial public offerings Initial Public Offerings is another type of equity financing that means the process of selling shares to the public with the help of secondary market. Kangaroo Express thinks of expanding for demand of products on satisfactory terms. This particular organization can opt for IPO for raising capital from the public (Collier 2016). It is important to consider the fact that Kangaroo Express needs to be assured regarding demand of the products before launching initial public offerings. In this case, risk is high as well as returns percentages at the same time. Long term bank loans, Asset-backed mortgage loans and Government bonds Long-term bank loans, asset-backed mortgage loans as well as government bonds are types of debt financing. Kangaroo Express can opt for long-term loans, as it will help in raising funds in smoother terms. It is slightly less risky process as it increases total debt amount from inherent risk association (Warren, Reeve and Duchac 2013). This particular aspect affects the capital structure of the given business organization. It is advisable to the business firm for obtaining certain portion of capital especially from financial banks. Business organization should make use of retained earnings for paying off interest in association with bank loans. The case study company is medium-sized and it is expected that the business form will have considerable amount of retained earnings for making it a profitable organization (Chen, Tan and Wang 2013). In this case, risk will be less and more return for Kangaroo Express. On the contrary, Kangaroo Express can even opt for government bonds. This is because government bonds are less risky as well as higher return on other side. After analysis both equity and debt financing, it is recommended that kangaroo Express should opt for bank loans as well as government bonds for raising capital for expansion purpose (Anderson et al. 2015). Problem 3 Ethical behavior is very important for managerial accounting. Ethical behavior in accounting largely affects relationship between company, employees and suppliers. Management accounting manages with the internal process of business firm so accountants need to abide with the norms of ethics in carrying out operations (Collier 2016). The major responsibility of an accountant is to present correct value in form of financial information on behalf of top-management of accounting firm. Ethical principles need to be followed by accountants based upon responsibility and objectivity. Management is liable to take strict actions on the accountants who present wrong or misleading information in the financial statements. At the time of external audit, if external auditors will find out some faults in the financial statement then top management will be questioned regarding any unethical decisions undertaken by them. There also occurs various legal consequences for given business accounting firm. There is wide-impact of ethics on relation with company as well as its stakeholders. Collier (2016) argues the ethical behavior largely affects relationship of the company with its key stakeholders. Most of the business organization needs to be accountable on behalf of its stakeholders. Business organization will be legally punished if they produce any kind of wrong information especially in the financial statements. Wrong information produced by business organization lowers down the goodwill that hampers the relationship with internal as well as external stakeholders. This includes customers as well as suppliers and employees (Collier 2016). Hence, management accountants need to follow various ethical standards while working for given business organization. Problem 4 This particular problem requires discussion on strengths as well as weakness of financial statements for determination of growth potential of suppliers. It is opined by Kaplan and Atkinson (2015) that financial statement analysis posses various strengths and weakness for determination of future prospects from supplier or any other business organization. Some of the major strength of financial statement is explained below with proper justification: Profit and Revenue Identification- It is important to understand the fact that financial statement depicts total revenue and total profit as earned by business firm for specified time. It can be considered as growth prospective from the point of view of the supplier as judged by sales as well as profit figures generated by business organization (Warren, Reeve and Duchac 2013). Cash flow statements and budgeted figures- It has been understood that financial statements helps in identification of total amount of cash inflows as well as cash outflow for determining the cash balance for any business accounting firm. Hence, it is easy to judge the supplier stability position by using financial statement analysis. Some of the weakness of financial statements is listed below with proper justification: Market condition- This is one of the weaknesses of financial statements as it fails in reflecting the probable market condition whereby accounting firm operates in given form. Supplier growth potential cannot be analyzed by using financial statements of a particular business organization. Continuity in operations- Financial statements fails in reflects continuous analysis on the sales as well as profit figures (Warren, Reeve and Duchac 2013). These figures are not presented in the financial statements of any business organization. Financial statement fails in determining the stability of the firm in proper way. Learning from standard set of financial statements It is important to consider the fact regarding the financial statements for acting as balanced scorecard for particular business firm for specified year (Libby, Rennekamp and Seybert 2015). It depicts on how much profit will be earned for given time. It helps in highlighting the cash position as well as total assets and liabilities for given business organization. Addition to that, financial statement reflects in rendering quantitative information that gives true as well as far valuation for particular business enterprise. Limitations of Financial Statements There are various limitations of financial statements that need to be addressed while preparation of the statement for particular business organization financial statement gives only quantitative information and completely ignores qualitative information (Warren, Reeve and Duchac 2013). Financial statement takes into consideration facts from historical costs. It fails to include market value of assets and liabilities in the financial statements (Libby, Rennekamp and Seybert 2015). Financial statements are majorly subjected towards fraudulent activities. Non-financial information are completely ignored by the financial statement users. Suppliers ability judgment Suppliers ability can be easily judged by cash flow statement as well as liquidity position of the concerned suppliers. Debt amount will give true picture regarding the suppliers for remaining in the business or not. In case debt amount of supplier is one the higher side, it will lead to weak capital structure (Warren, Reeve and Duchac 2013). This will lead to cash flow problems in the upcoming financial years. Additional capital for growth purpose It is easy to assess the capital structure by understanding the relationship between debt as well as equity. In case of stable relationship, company has the liability in opting for additional capital growth (Libby, Rennekamp and Seybert 2015). Business firm can attract additional capital for future growth in case there is positive relationship between cash flow position as well as liquidity ratio at the same time. Conclusion At the end of the study, it is concluded that there are major four problems highlighted in the assignment. It is in relation with finding out the importance as well as limitations of management accounting for given business enterprise. Organizations are liable in undertaking several decisions after analyzing financial statements. It is required for the accountants to follow the ethical standards of financial statements. It will increase the sustainability of business firm if every accountant follows the ethical standards while preparing the financial statements. Reference List Anderson, S.B., Brown, J.L., Hodder, L. and Hopkins, P.E., 2015. The effect of alternative accounting measurement bases on investors assessments of managers stewardship. Accounting, Organizations and Society, 46, pp.100-114. Chen, W., TAN, H.T. and Wang, E.Y., 2013. Fair value accounting and managers' hedging decisions. Journal of Accounting Research, 51(1), pp.67-103. Collier, P., 2016. Accounting For Managers Interpreting Accounting Information For Decision Making 0470845023. Collier, P.M., 2015. Accounting for managers: Interpreting accounting information for decision making. John Wiley Sons. Dhamija, S., 2015. Financial Accounting for Managers, 2/e. Pearson Education India. Droms, W.G. and Wright, J.O., 2015. Finance and accounting for nonfinancial managers: All the basics you need to know. Basic Books. DRURY, C.M., 2013. Management and cost accounting. Springer. Guo-liu, H.U. and Sui, Z.H.O.U., 2013. Accounting conservatism, managers overconfidence and excessive investment [J]. Journal of Southeast University (Philosophy and Social Science), 2, p.015. Hartley, W.C., 2014. An introduction to business accounting for managers. Elsevier. Healy, P.M. and Palepu, K.G., 2012. Business Analysis Valuation: Using Financial Statements. Cengage Learning. Horngren, C.T., Sundem, G.L., Schatzberg, J.O. and Burgstahler, D., 2013. Introduction to management accounting. Pearson Higher Ed. Kaplan, R.S. and Atkinson, A.A., 2015. Advanced management accounting. PHI Learning. Libby, R., Rennekamp, K.M. and Seybert, N., 2015. Regulation and the interdependent roles of managers, auditors, and directors in earnings management and accounting choice. Accounting, Organizations and Society, 47, pp.25-42. Warren, C.S., Reeve, J.M. and Duchac, J., 2013. Financial managerial accounting. Cengage Learning.

Monday, December 2, 2019

International students consume alcohol Essay Example

International students consume alcohol Essay Restatement of the research objective The research focuses on drawing a comparative analysis between Australian and the international students drinking habits. Overall, the data shows that overseas and Australian students consumption of alcohol is quite different. As this research shows, on average both international male and female students consume alcohol at least once a month. On the other hand, the Australian students drink alcohol mainly two days a week. In addition, beer is the conceivably the most common drink among the international students while brand choice is evidently differentiated among the Australian students. The majority of Australian female students consume bottled wine while their colleague males predominantly drink beer irrespective of their alcoholic strengths. We will write a custom essay sample on International students consume alcohol specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on International students consume alcohol specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on International students consume alcohol specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Research findings From the data obtained in this study, the first finding is that that alcohol is definitely the highest indulgence drug among both Australian and the international students. Beers and spirits account for the highest proportion of alcohol used by the international students while higher incidences of bottled spirit use and beer drinking is reported among Australian female and male students respectively. Most male international students drink at least twice a week while international female students only drink occasionally. Conversely, a greater proportion of the Australian students drink mainly two days a week. One probable reason for higher incidences of alcohol use among male than female students is the contemporary youth mentality that associates drug use with masculinity. Moreover, the society assigns females the role of caregivers hence they embrace sobriety early enough in their life. The second finding of this research is that taste of drink highly influences the brand relished by the international students than it is the case with Australian students. While both the male and female the international students exhibit an almost equal liking for beer, the Australian male students are common drinkers of wine while females relish bottled wine. Therefore, it .means that beer has the most favorable taste than all the other drinks that the international students choose from. On the other hand, there is a marked difference between the drinking of alcohol among international and the Australian students. The probability that psychological attunement of the females towards details such as taste and brands is higher than that of the males. Therefore, males would only consider a few factors before settling on a brand of choice as opposed to the females. While disposable income among the Australian students is the most important determinant of their respective drinking habits, taste is the most significant determinant of the brand choices among the international students. Due to the differential prices of various types of wine, its use among the Australian students is the most segmented with lower income students less predisposed to drinking cask wine than the medium to high income ones. Price only has an overreaching influence on the brand choices of the international female students. The most plausible reason for the influence of pricing in the Australian students choice of alcohol brand is their limited income. However, overseas students mainly come from affluent backgrounds thus are able tis sustain their alcoholic lifestyles devoid of price limitations. This research determines that a larger proportion of female international student drinkers consume alcohol at least once a week, at least once a month and less than once a month as compared with their male counterparts whose drinking habit is not dependent on time factors. The international male students drink alcohol at least twice a week is higher than female. These findings indicate the high levels of caution that female international students employ to prevent binge drinking or becoming alcohol addicts. Drinking of beer among the Australian male drinkers is differentiated into cohorts with decipherable differences in brand choices between baby boomers, generation X and generation Y. While age is not a determinant in the international students brand choices, it is significant among Australian drinkers with baby boomers males drinking more spirits that beer. Among the international male and female students, beer is universally extensively drunk than any other brand irrespective if the drinkers age. The choice to drink only occasionally reflects the resilience of both the international and Australian female students to the effects of group pressure compared to their male counterparts. In addition, the higher rates of drinking among both overseas and Australian male students can be attributed to the fact that males are more susceptible to group influence, especially with respect to drug use than the females are. Limitations and recommendation of the study The research does not provide personalized information that influence differential alcohol use among the international male and female students respectively as it does for the Australian. Factors such as societal expectations and resistance to peer pressure among female students would be rather compelling assertions of the research. Therefore, future research should focus on the social factors that influence the differentiated drinking trends between Australian and overseas students.

Tuesday, November 26, 2019

Leadership and Management Development Essays

Leadership and Management Development Essays Leadership and Management Development Essay Leadership and Management Development Essay Leadership and Management Development Name: Institution: LEADERSHIP AND MANAGEMENT DEVELOPMENT Introduction Leadership and Management development are important in the successful operation of any organization. This report details several items on my agenda as the new Management and Development programs Director at the NSCA. First, this report will highlight the framework that I will adopt in the new position. It will also suggest the policies I will implement in my new capacity. The report will identify processes crucial for the NSCA’s success, as well. Finally, this report will identify a few programs that this office shall commence. All the strategies that identified in this report are to ensure that the NSCA has the right management, workforce and enough resources, to ensure effective operation. 1.) Leadership Framework A leadership framework dictates that leadership should not be limited to personnel holding traditional leader or management roles. It also states that leadership in an organization is most effective when we divide responsibilities in service delivery. At the NSCA, any staffer can therefore provide leadership. This statement holds for as long as they illustrate appropriate mannerisms. Such mannerisms are empowering the leadership levels of fellow staffers and significant contributions to the leadership process (Hargreaves Fink, 2006). For effective implementation of the leadership framework in this organization, several changes are required. For starters, the workforce needs restructuring. It is my recommendation that; first, personnel be divided into several teams. At the helm will be a team leader/ supervisor. The team leader’s responsibilities include; providing clear direction to the team, allocating work to team members and resolving conflicts within the team satisfactorily. They will also be required to provide the team and its members with a supportive and inclusive environment. Above the team leaders, Managers will be in charge. The managers will have various roles as well. A few are; delegating responsibilities to the team leaders, monitoring team performance and taking necessary action, supporting the teams create and maintain team values, and developing the staff below them. It is my recommendation for the placing of Senior Managers above Managers at the NSCA. This hierarchy will be necessary in limiting overlapping responsibilities. The proposed Senior Managers should hold several responsibilities. First, they should communicate the departmental objectives to the staff and teams below them. They should monitor and review performance of managers and staff under them. Finally, they should be able to act as mentors to the staff. In this role, they will provide specialist guidance and support to the respective department (Steers, Sanchez-Runde Nardon, 2010). At the helm of each department, it is my recommendation that a Director takes charge. Such leaders are ‘Strategic Managers.’ Their main role will be providing effective leadership in their areas of responsibility. They will also be required to create and announce the strategic direction for their areas of jurisdiction. In the organization, they should be able to promote an environment where diversity is highly valued. Strategic managers will be finally required to act as mentors to staff under their leadership. The leadership model identified above describes a distributed or shared model. Everyone in the NSCA has the opportunity to contribute to the leadership process, without necessarily being a leader. This is through the responsibilities described in the Leadership Framework. 2.) Leadership Management Policies The purpose of leadership is to develop vision, commitment, and embracing innovation among staff. Management, on the other hand, develops systems that improve consistency and predictability in service delivery. Effective leadership and management are important due to a variety of factors. First, they enable the organization to carry out its national priorities, successfully. Secondly, they provide learning environments for staff. Such communities are typically safe, ambitious and active. Effective leadership and management enable the organization in developing changes and improvements necessary for perfecting it. Lastly, they enable staff to provide excellent service consistently. The aforementioned policy statement results into several important principles. The first is promotion of leadership at all Organisational levels. This will widen the existing range of experiences and skills, existing in the organization, to all staff. The next principle dictates that; the NSCA will provide a framework for efficient and sustained support in leadership and management to all staff and teams. The organization shall strive to provide a program of support in leadership and management. The aforementioned program requires placing in a structure; understood and implemented by all staff. The prospective Leadership and Management policy ha several outcomes expected out of it. Leaders and managers working within legal, financial and national influences, have several things expected out of them. They are required to uphold and support the strategic vision, values and aims of the National Civil Service Agency (NSCA). They should embrace and grow the team approach, structured into service delivery. Finally, they will be required to nurture sustained development and improvement of staff and the services provided to the Public. To ensure that implementation of the aforementioned statements, each member of staff at the NSCA has responsibilities. These responsibilities are employee responsibilities and managerial responsibilities. Employees have a duty to perform in a professional manner, uphold the Leadership and Management policy statement and to recognize, respect and uphold individual roles and duties in the organization. Managers also have responsibilities to carry out: they will be required to appropriately listen to their employees and peers and act effectively. Managers are required to promote the participation of teams and their members in developmental activities. Finally, they are required to provide direction to the teams under their leadership. 3.) Leadership processes. There are two key leadership processes in existence. For the NSCA’s effectiveness in service delivery, delivery of both needs implementation without fail. The leadership processes include management and vision. Vision describes where leadership begins. It is an image of the future desired by the organization. In short, vision guides the organization’s progress. Management describes the ability to implement objectives, by planning, organizing and effectively employing resources. Management is associated with prudence and preserving limited resources. Managers lacking vision may limit innovation in the organization, while, leaders lacking managerial skill may lose the organization’s resources (Raadschelders, Toonen Meer, 2007). 4.) Leadership and Management Programs To ensure the success of the NSCA in Leadership and Management, several programs need implementation. First, mentorship programs need implementation in the organization. Such a program will enable staff to take part in leadership activities, directly. Managers need to provide them the opportunities to make decisions and see their effects. As a result, employees can manage lower duties, while, managers provide leadership where necessary. Short Leadership and training courses also need to implementation, in partnership with an appropriate higher learning institution. This will enable employees and managers to gain the latest information on effective leadership and management programs. As a result, employees and team leaders will gain fundamental leadership skills suited for their work environments. Managers in turn receive skills necessary in improving the organization’s productivity. 5.) Conclusion Effective leadership is a crucial aspect of public governance. It leads to enhanced management capacity and performance in public institutions such as the NSCA. The carrying out of change in Leadership and Management is paramount. If not, public service organizations will fail in reforming their service delivery. Various studies have found that there is a strong link between effective leadership in public organizations and their performance. It is my desire that full implementation of all strategies mentioned in this report is carried out. References Hargreaves, A., Fink, D. (2006). Sustainable leadership. San Francisco: Jossey-Bass Raadschelders, J. C. N., Toonen, T. A. J., Meer, F. M. (2007). The civil service in the 21st century: Comparative perspectives. Basingstoke: Palgrave Macmillan. Steers, R. M., Sa?nchez-Runde, C., Nardon, L. (2010). Management across cultures: Challenges and strategies. Cambridge: Cambridge University Press. Topss England., HZ Management and Training Consultancy. (2004). Leadership Management. Leeds: Topss England.

Saturday, November 23, 2019

How Do You Calculate SAT Score Raw and Scaled

How Do You Calculate SAT Score Raw and Scaled SAT / ACT Prep Online Guides and Tips You know your SAT score is important for college admissions and even things like scholarships, but how does your SAT score get calculated? I'll show the steps to calculating your final SAT score so you can get an accurate idea of how well you're doing on the exam. // Step 1: Determine Your Raw Scores Your raw score is simply calculated using the number of questions you answered correctly. // For every question you answer correctly on the SAT, you receive one point. There is no penalty for guessing or skipping. // The maximum possible raw score varies by section (and depends on the total number of questions asked). For example, for the Reading Test, there are 52 questions, so the maximum raw score is 52. If you answered all 52 questions correctly, you would have a raw score of 52. For Math, there are 58 questions. For Writing, there are 44 multiple-choice questions. // There is one essay, which is graded separately on a scale of 2-8 and is not factored into your composite score (your 400-1600 score); therefore, I will not be discussing it further in this article, but for more information, read our articles on the new SAT essay prompts and the SAT essay rubric. // Step 2: Convert the Raw Scores to Scaled Scores The raw score is converted into the scale score (on the 200 to 800 scale for each section) using a table. This table varies by SAT test date. The table is used as a way to make sure each test is â€Å"standardized†. The table is a way of making â€Å"easier† SAT tests equal to the â€Å"harder† SAT tests. For instance, a raw score of 57 in Math might translate to an 800 on one test date and 790 on another. // For Math, you simply convert your raw score to final section score using the table. For the Evidence-Based Reading and Writing section score, there is an extra step. You get individual raw scores for the Reading Test and the Writing and Language Test. These two raw scores are the converted into two scaled test scores using a table. The two test scores are then added together and multiplied by 10 to give you your final Evidence-Based Reading and Writing section score (from 200 to 800). I'll explain this more in-depth with examples below: // You cannot know what the raw to scale score conversion will be in advance. While the exact raw to scale score conversion will vary by testing date, the College Board supplies this example chart in their new SAT Practice Test: Raw Score Math Section Score Reading TestScore Writing and Language Test Score 58 800 57 790 56 780 55 760 54 750 53 740 52 730 40 51 710 40 50 700 39 49 690 38 48 680 38 47 670 37 46 670 37 45 660 36 44 650 35 40 43 640 35 39 42 630 34 38 41 620 33 37 40 610 33 36 39 600 32 35 38 600 32 34 37 590 31 34 36 580 31 33 35 570 30 32 34 560 30 32 33 560 29 31 32 550 29 30 31 540 28 30 30 530 28 29 29 520 27 28 28 520 26 28 27 510 26 27 26 500 25 26 25 490 25 26 24 480 24 25 23 480 24 25 22 470 23 24 21 460 23 23 20 450 22 23 19 440 22 22 18 430 21 21 17 420 21 21 16 410 20 20 15 390 20 19 14 380 19 19 13 370 19 18 12 360 19 17 340 17 16 10 330 17 16 9 320 16 15 8 310 15 14 7 290 15 13 6 280 14 13 5 260 13 12 4 240 12 3 230 10 2 210 10 10 1 200 10 10 0 200 10 10 // Note: this is just an example. The exact conversion chart will vary slightly depending on the individual test. Why are Reading and Writing and Language listed as separate sections? Why are they graded from 10-40 instead of 200-800? As I mentioned briefly before, you get separateraw scores for the Reading and Writing and Language. You then take these two raw scores andconvert them into two scale scores using the above table. For example, if you answered 33 correctly in Reading and 39 correctly in Writing and Language, your scale scores would be 29 and 35, respectively. These two scaled scores are then added together and multiplied by 10 to give you your finalEvidence-Based Reading and Writing section score (from 200 to 800). Continuing the above example, if your scale scores were 29 for Reading and 35 for Writing and Language, your final Evidence-Based Reading and Writing scaled score would be: (29 + 35) x 10 = 64 x 10 = 640 Step 3: Take the Scaled Scores and Add Them Together Once you have your scaled score for both the Math and Evidence-Based Reading and Writing sections, you just add them together to get your overall SAT composite score. For example, if you scored a 710 in Math and 640 in Evidence-Based Reading and Writing, your composite score would be 710+640 = 1350. // How to Understand Your SAT Score Report The College Board gives you the breakdown of your incorrect, correct, and omitted answers on your SAT score report in addition to your final scaled scores. See below excerpts from a real new SAT score report: Note that on this test, the raw Math score was out of 57, not 58, points. This sometimes happens when a question on the test is deemed to be unfair or unanswerable and the SAT drops it from everyone's scoring. For the Reading and Writing and Language sections on this SAT score report, this student’s raw scores were 52 and 42. These raw SAT section scores scaled to section scores of 40 (Reading) and 39 (Writing and Language), which translated to a 790 Evidence-Based Reading Writing Score: (40 + 39) x 10 = 790 I'd like to emphasize that you will not be able to determine what the full table of raw to scaled scores conversion was from your score report. Instead, you will only be able to determine what your raw score was and see how it translated to your scaled score. What This Means for You Once you have determined your target SAT scorein terms of raw score, you can use it to determine your SAT test strategy options.We have plenty of resources to help you out. Once you know what SAT score you're aiming for and how far you are from that goal score, you can begin to develop a study plan, gather study materials, and get to work on raising your score! If You Need Help Creating a Study Plan How to Build an SAT Study Plan How to Cram for the SAT How Long Should You Study for the SAT? If You Need More Study Materials Complete Official SAT Practice Tests The Best SAT Prep Books The Best SAT Prep Websites You Should Be Using If You Want to Raise Your Score The Best Way to Review Your Mistakes for the SAT How to Get an 800 on SAT Reading How to Get an 800 on SAT Math What’s Next? Want to rock the SAT? Check out our complete SAT study guide! Want to find free new 2016 SAT practice tests? Check out our massive collection! Not sure what score to aim for on the new SAT? Read our guide to picking your target score. Disappointed with your scores? Want to improve your SAT score by 160 points?We've written a guide about the top 5 strategies you must be using to have a shot at improving your score. Download it for free now: Have friends who also need help with test prep? Share this article! Tweet Dora Seigel About the Author As an SAT/ACT tutor, Dora has guided many students to test prep success. She loves watching students succeed and is committed to helping you get there. Dora received a full-tuition merit based scholarship to University of Southern California. She graduated magna cum laude and scored in the 99th percentile on the ACT. She is also passionate about acting, writing, and photography. Get Free Guides to Boost Your SAT/ACT Get FREE EXCLUSIVE insider tips on how to ACE THE SAT/ACT. 100% Privacy. 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Thursday, November 21, 2019

What evidence is there of application of the materiality concept in Research Paper

What evidence is there of application of the materiality concept in the report - Research Paper Example The most conspicuous evidence of the materiality principle in the preparation of JB Hi Fi’s financial report is minimal disclosure overload. According to the International Accounting Standards (IAS), most of the listed companies are expected to follow the structure as presented in paragraph 114 of IAS 1. However, JB Hi Fi is a conglomerate and deals with large volumes of financial information. Therefore, the company deals with complex financial information that requires sophisticated and alternative structures of presenting financial information. The company’s structure is different from the one proposed by international accounting standards, and better communicates the links between different pieces of information. Consequently, the company has enhanced its reflection of the financial position as well as the risks associated with the operation of the business. IAS proposes that listed companies should list intangible assets followed by trade and other receivables and finally trade and other payables when preparing the balance sheet. However, JB Hi Fi has a different order of items when preparing its balance sheet. The company lists trade and other receivables, intangible assets and trade and other receivables in an ascending order. JB Hi Fi bases its list of the balance sheet items on the importance of information presented in the financial report. Furthermore, the company cautions users of financial information not to read the financial statements in isolation. Rather, the company suggests that users of financial information should analyze financial statements in additional to reading notes to financial statements. The company’s statement of financial position has the three standard categories including assets, liabilities and equity. Each of these categories contains information about items related to each other for the sake of

Tuesday, November 19, 2019

Evolution of Rights and Responsibilities as Compared Between Native Essay

Evolution of Rights and Responsibilities as Compared Between Native American, African American and European American Women in th - Essay Example Tracing the social organization of Native American societies in the 18th century, Theda Perdue observes that these cultures practiced a well delineated division of labor between the sexes. She gives the example of the Wahpeton Dakota community where men and women lived three months of each year apart as the former hunted muskrats while the latter was involved in the production of sugar (Perdue 1999). Such a gendered division of labor, though perhaps not in consonance with modern notions of equity, ensured to Native American women a considerable degree of social autonomy, that which was denied to them during the heyday of the colonial era. Perdue thus refers to Bernard Romans to illustrate the manner which racial and gender intolerance mingled to in the manner in which Native American women were portrayed in the accounts of the early European settlers- â€Å"Their strength is great, and they labor hard, carrying very heavy bundles a great distance; they are lascivious, and have no id ea of chastity in a girl† (Romans qtd in Perdue 1999). The above description points to the collusion between racial and gender stereotypes whereby Eurocentric prescriptive notions of femininity were superimposed upon Native American women. The most striking shift in power equations could be seen in the case of the Cherokees. Anthropologists observe that Cherokee women had equal ownership of land and property, a practice that changed with the tribe being forced to sell significant portions of their land holdings to the United States government. The colonial government entered into these agreements with the men of community, rendering at once, the traditional parity of gender in these groups awry and creating a distinct hierarchy between the sexes (â€Å"Native women are fighting for their rights – and their lives† n.d.). This rupture within the community was deepened by then Voting Rights laws put forth for Native Americans by the American government. Till 1924, t ribes were often compelled to abandon their social and cultural affiliations with their respective groups in order to gain the right to vote in American elections. Yet again, the men already rendered more powerful due to superior economic rights had a more pronounce incentive in giving up their tribe affiliations (â€Å"Native women are fighting for their rights – and their lives† n.d.). The Indian Reorganization Act of 1934 was enacted chiefly to acknowledge the contribution of Native American troops in World War I. The act led to the reduction of federal involvement in Native American community life and increased the role of tribal self-governance. Though it was unprecedented in the autonomy that it conferred upon the Native Americans within the USA, the act also consolidated their â€Å"outsideness† and hence was not equally well received across Native American communities. Furthermore, the restoration of land to Native Americans yet again accentuated gender inequalities as it did not ensure equitable distribution between the sexes

Sunday, November 17, 2019

Wealth over Health Essay Example for Free

Wealth over Health Essay People tend to look for wealth forgetting about their health and mostly after they have gained their wealth cannot save their health. Pictures tend to convince as to do the unimaginable just because hey mostly encourage as to what we think we are not capable of doing. The picture above clearly implies that we should help save a life but has a deeper meaning of speaking to people who spend all their time working to earn money and then think they have it all in life. Here I believe the image achieves it’s purpose because it stirs up a feeling to help donate blood because it makes you empathize with individuals who are in the same situation and the inscription on the image makes anyone think beyond what the image portrays so here we see that though we are being emotionally convinced we are being provoked to think about wealth and health and not just donating blood. Pictures appeal to pathos, logos and ethos which tend to one way or the other speak to everyone. As individuals of a technological age most of our decisions are influenced by the images we see around us because what we see tends to draw our attention and speaks to us in different ways. I believe images have the ability to change our mood, how we feel and how we think. The image above can be classified in having the ability of changing a persons mood from happy to sad or sad to happy. A person earning a lot of money who hasn’t thought about their health might be sad and a healthy person earning an average amount will be very excited to help save a life. Most of the time we create meaning through stories images project in our mind and they also tend to remind us of sad or happy moments we have experienced. Pictures, videos and all forms of visual imagery carry power which is able to convince us more than what we read or hear on radio. With the picture above we see blood and it make anyone alarmed to see what follows because as human beings blood is one of the most important things we need in our bodies since it helps  regulates the functions of all our organs. Here, the blood could have filled the container to create an image of shortage of blood in our mind which is a form of persuasion to help you save someone who is loosing blood. On the inscription on the poster it says, â€Å"save 3 lives without spending a cent† comparing three human lives to a cent just to show that money cannot help in all cases but as a human being you should know you are more important than money and so don’t spend all your time trying to get it but just spend a little of your time donating to someone who needs a little of your blood. I believe the red cross on the poster can also be speaking to Christians who believe Christ died on cross and shed His blood for healing. We are easily influenced by images because they tend to illustrate the unimaginable and give us confidence. In the image above we see ourselves as life savers due to the power the image creates in our mind and we now forget the pain of the needle being passed through our skin to get the blood out. We easily feel good because images persuade us to do the impossible and see the invisible. Most phrases we hear on our TV ads are â€Å"Look, it’s easyâ⠂¬  or â€Å"Microwave for 10 minutes and you just made the best meal’’. We feel impressed and have a sense of accomplishments when we see such words speaking to us in the form of images. The main purpose of images is to make us want to think we can do something which we don’t believe is possible and persuades us further that we can do it. In the second image it speaks clearly without the help of words. If you don’t take good care of your health whiles you have money you spend all your wealth on different kinds of medicines. This can also be a mild way of communicating to a particular audience that money is not everything because you can be purchasing medicines either to prevent a disease or cure a disease but you cannot be guaranteed an assurance on better health. In the article Health is Wealth and Wealth is Health – perceptions of health and ill health among female workers in Savannahkat Laos , â€Å"Health was defined as a condition that is needed to be able to work and collect money.† I think this definition was used because the sex workers need to get money to take care of their health. The amazing thing about images is that they are able to relate to each and everyone just like the pictures above. Most images are successful because they tend to persuade, inform and advertise to a large group of people. We make decisions based on what we see and though some images have a deeper meaning they are able to speak and achieve their aim of persuasion. We see through both images that we don’t only have to focus on our wealth and exclude our health but focusing on both will make you happy and will also make society a great place to be. Our health and wealth are very important and without both the journey of life is a tough one. Visual images find themselves in different contexts relating to different people but to make your point clear one needs to make an image have a connection with every individual and carry power to change the mood ,thought or action of a peron.

Thursday, November 14, 2019

Essay --

Over the years, our accounting system has evolved from the tedious task of manual accounting to the ease of computerized accounting systems and programs. Computerized accounting has brought about a more efficient way of setting up and maintaining a company’s financial records. In previous years accountants would spend copious amounts of time manually recording business transactions through journalizing, posting to ledgers and mathematically checking each journal and ledger for errors. This process was necessary in order to compile data needed to produce financial reports and statements. The number of manual entries were so numerous that the margin of error was significantly increased. Simple mistakes, such as transposing a number or incorrectly recording a transaction in the wrong column could create a ripple effect of errors throughout each journal, ledger, or report issued for that period. The process of tracking one simple sales transaction was incredible compared to today’s accounting software. Manually, the transaction might begin with an invoice or sales receipt which beg...

Tuesday, November 12, 2019

Ethical Issues in Research Essay

Ethical issues are essential factors that must be given due and proper consideration in research. This becomes even more applicable when researchers deal with studies that involve human beings and animals. Numerous experiments are being made to achieve progress in various fields of discipline, including psychology. In doing so, human beings and animals must be protected, especially since using them in these experiments involves their well-being. For this reason, ethical standards are established. Ethical standards serve as the guidelines that govern the proper and improper participation and utilization of these life forms in research (University of Alberta, 2008). These standards ensure that studies which involve human beings and animals are subjected to limitations so that they would not be taken advantage of. According to the British Psychological Society (BPS) (2008), ethical guidelines give importance to respecting an individual as the researchers should not express any sign of prejudice toward the participants due to differences in racial descent, sexuality, language, and such. The privacy of the participants should also be respected. Thus, researchers must only acquire the personal information necessary for the study, and this information must be kept confidential. The researchers should also obtain the consent of the individuals who will participate in the study. During the study itself, participants should also be protected from any kind of harm, may it be psychological (e. g. , stress and anxiety) or physical. Moreover, the researchers also have to respect the participant’s right to leave an experiment any time that he or she wishes to. In another perspective, ethics tend to limit the advancement of psychological science. Since there are many guidelines and rules that have to be followed, researchers do not have full control over their participants. This sometimes hinders and impedes the experimentation process. Furthermore, obtaining the necessary license for this kind of research could be time-consuming, which can be used for the study itself (BPS, 2008). Scientists should accept and follow the limitations brought about by ethical guidelines because these guidelines only give due importance to the life of the participants. The main justification for these research studies is that the findings obtained from these would contribute to the knowledge and information on various fields of discipline and help improve the lives of individuals. This gives such studies an objective that is morally good (National Academy of Sciences, 2006). Hence, scientists should value the lives of the people they claim to help. Lastly, the one responsible in identifying the parameters of the participants’ protection are competent organizations that are familiar with the field of study being researched. In the case of the United States, the American Psychological Association (APA) (2008) is an organization that represents the psychologists in the U. S. APA (2008) implements an ethics code that every member of the organization must adhere to. References American Psychological Association. (2008). APA Ethics Office. Retrieved September 15, 2008, from http://www. apa. org/ethics/.British Psychological Society. (2008). Ethics. Scienceaid. co. uk. Retrieved September 15, 2008, from http://scienceaid. co. uk/psychology/approaches/ethics. html. National Academy of Sciences. (2006). Scientific and ethical justification for using animals in research. Online Ethics Center for Engineering. Retrieved September 15, 2008, from http://www. onlineethics. org/cms/16216. aspx. University of Alberta. (2008). Human Ethics, Biohazards, and Animal Welfare. Retrieved September 15, 2008, from http://www. rso. ualberta. ca/certification. cfm.

Sunday, November 10, 2019

Svrwvrev

LOVELY PROFESSIONAL UNIVERSITY CAPSTONE PROJECT REPORT TOPIC- ANTIMICROBIAL ACTIVITY OF DIFFERENT TYPES OF HONEY. PROJECT GUIDE- SUBMITTED BY- DR. AKSHAY GARG MOHIT KUMAR DEPT. OF BIOTECHNOLOGY REG. NO. – 10800037 ROLL NO- RB1R07B02 B. TECH BIOTECH. (8th sem. ) DATED- . 17-05-2012 CERTIFICATECertified that this project entitled â€Å"anti microbial activity of different types of honey † submitted by MOHIT KUMAR , students of biotechnology Department, Lovely Professioal University, Phagwara Punjab in the partial fulfillment of the requirement for the award of Bachelors of Technology (biotechnology) Degree of LPU, is a record of student’s own study carried under my supervision & guidance. This report has not been submitted to any other university or institution for the award of any degree.Date: 17/5/2012 Name of Project Guide Dr. Akshay Garg DECLARATION I, MOHIT KUMAR, student of B. Tech Biotechnology under Department of Biotechnology of Lovely Professional Unive rersity, Punjab, hereby declare that all the information furnished in this dissertation / capstone project report is based on my own intensive research and is genuine. This dissertation / report does not, to the best of my knowledge, contain part of my work which has been submitted for the award of my degree either of this niversity or any other university without proper citation. Date – 17/5/2012 Investigator- MOHIT KUMAR Regd. No. 10800037 Acknowledgement Any attempt at any level can't be satisfactorily completed without the support and guidance of learned people in my capstone projectâ€Å"Antimicrobial activity of different types of honey†.I would like to express my immense gratitude to my guide Dr. Akshay Garg for his constant support and motivation that has encouraged me to come up with this project. I also would like to thanks my group member who helped me in my project. MOHIT KUMAR ABSTRACT Honey is a traditional topical treatment for infected wounds. It can be effective on antibiotic-resistant strains of bacteria. Several local brands of honey(Dabur) collected from different sources is used in this study. Also the honey is collected from various apiaries from Pathankot(Punjab) and Saharanpur (Up).The bacterial culture were taken from the laboratory of the Lovely professional University and was revived in nutrient broth media and then sub-cultured in nutrient agar media. The method used to test this antibiotic sensitivity of honey was done with the help of Kirby Bauer method . Marked variations were observed in the antibacterial activity of these honey samples. Bacterial species used were Bacillus subtilis ,E. coli. , S. aureus and Burkholderia spp. The zone of inhibition produced against different bacteria using honey of different concentration are as follows: E. oli (pathankot sample): 100% = 2. 6cm, 75%= 1. 9cm, 50%= 1. 7cm,and 25%= 1. 2cm; E. coli (dabur sample): 100% = 2. 2cm, 75%= 1. 6cm, 50%= 1. 3cm, and 25%= 1. 1cm; E. coli (sahar anpur sample): 100% = 2. 8cm, 75%= 2. 4cm, 50%= 2. 2cm, and 25%= 1. 7cm; B. subtilis (pathankot): 100% = 3. 0cm, 75%= 2. 7cm, 50%= 2. 7cm, and 25%= 2. 5cm; B. subtilis (dabur): 100% = 2. 7cm, 75%= 2. 5cm, 50%= 2. 4cm, and 25%= 2. 2cm; B. subtilis (saharanpur):100% = 3. 2cm, 75%= 2. 7cm, 50%= 2. 3cm, and 25%= 1. 5cm; Burkholderia spp. (pathankot): 100% = 2. 1cm, 75%= 1. 9cm, 50%= 1. 6cm, and 25%= 1. 4cm; Burkholderia spp. dabur): 100% = 2. 4cm, 75%= 1. 7cm, 50%= 1. 5cm, and 25%=1. 2cm; Burkholderia spp. (saharanpur):100% = 2. 5cm, 75%= 1. 9cm, 50%= 1. 7cm, and 25%=1. 4cm; S. aureus (pathankot):100%=2. 1cm, 75%= 1. 7cm, 50%= 1. 4cm, and 25%=1. 2cm; S. aureus (dabur):100% =3. 0cm, 75%= 2. 7cm, 50%= 2. 2cm, and 25%=1. 8cm; S. aureus (saharanpur): 100% = 2. 9cm, 75%= 2. 5cm, 50%= 2. 0cm, and 25%=1. 6cm; Zones of inhibition of different treatment groups were measured by agar-well-diffusion assay and compared with control. The comparison of honey sample with distilled water control had pro ved it significant.CONTENTS PAGE 1. INTRODUCTION 7 1. 1) Honey as antibacterial agent 7 1. OBJECTIVE 9 2. LITERATURE REVIEWS 10 3. 1 TYPES OF HONEY 10 3. 2 COMPONENTS OF HONEY 11 3. MATERIALS AND METHODS 15 4. MATERIAL 15 4. 2 METHOD 16 4. RESULTS AND DISCUSSION 19 5. REFERENCES 32 1. ) INTRODUCTION Honey is a sweet food made by bees using nectar from flowers. The Honey is a sweet, viscous fluid produced by bees from the collection of nectar, primarily from flowers. It is considered to be a natural syrup. The Nectar is gathered by the bees and is slowly transformed into honey, through a long rocess involving the addition of enzymes and the gradual reduction of moisture. Honey is a rich source of carbohydrates mainly Fructose and Glucose. The chemical composition of honey varies depending on the plant source, season and production methods. Therefore the Colour, Concentration and Compounds vary depending on the floral sources. Other compounds which can be found in Honey include Pr oteins and acids such as Gluconic Acid (C6H11O7, also known as 2,3,4,5,6- pentahydroxyhexanoic Acid), Minerals and Anti-Oxidants such as Hydrogen Peroxide (H202) and Vitamins (B6 and B12), (BD.Yates et. al. 1996). Honey has a low pH and a low moisture content, which is usually on average about 17 percent. The Gluconic Acid in honey is produced when bees secrete Glucose Oxidase, while processing the nectar, this give honey a low pH. There are many varieties of honey from around the world which come in three main types which are liquid, whipped and comb. Several local brands of honey(Dabur) collected from different sources is used in this study. Also the honey is collected from various apiaries from Pathankot(Punjab) and Saharanpur (Up) .Each of these honey’s were chosen because they are organic and readily available in health food stores 1. 1) The Antibacterial Effects of Honey The honeys are shown to have antibacterial properties, in particular Manuka honey. Manuka Honey has had extensive research done on it. It has been shown in many studies that Manuka Honey has antimicrobial effects (Barret J. , et. al, 2005; Coumbes A. L. , et. al. 2004; Mundo, M A. 2004). The Mechanism of Antibacterial Properties Honey has many natural properties which enable it to inhibit bacteria.These properties include, a low pH which is in the range of pH 3. 2 to 4. 5, approximately 3. 9 which is due to its content of acidic compounds mainly Gluconic acid as stated above. A low pH is inhibitory to most bacteria. Since most bacteria live in environments around pH 7, the pH of honey could inhibit the bacteria (Barrett, J et. al. al 2005). This is because pH affects the way large proteins such as enzymes work. Which causes the shape of enzymes to change, which then alters the overall charge, this causes the protein to denature.Honey contains small amounts of Hydrogen peroxide (H202) this varies depending on the honey, it is produced as a result of the enzyme glucose oxidase activ ity in producing Gluconic acid (Mundo, MA. et. al. 2004). C6H12O6 + H2O + O2 > C6H12O7 + H2O2 (glucose oxidase reaction) When honey is used topically (as, for example, a wound dressing), hydrogen peroxide is produced by dilution of the honey with body fluids. As a result, hydrogen peroxide is released slowly and acts as an antiseptic. Hydrogen peroxide is a powerful oxidising agent (Free Radical) which has the ability to damage cells.In an aqueous solution hydrogen peroxide acts like an acid and can oxidise a variety of compounds, by accepting free unpaired electrons. This allows the formation of other free radicals, which then causes a cascade effect. Therefore altering biological structures and therefore damaging cells. Honey is primarily a saturated mixture of two monosaccharide, with a low water activity; most of the water molecules are associated with the sugars and few remain available for microorganisms, so it is a poor environment for their growth.If water is mixed with hone y, it loses its low water activity, and therefore no longer possesses this antimicrobial property. Honey consists of various constituents such as water, carbohydrates, proteins, vitamins, amino acid, energy and minerals. Besides the major ones, there must also be several minor constituents in honey, which may be playing a key role in determining the antimicrobial behaviour of honey. In the past, antimicrobial activity of honey had been reported only by using aqueous solution of honey.It is said that honey possesses antibacterial property but it is not clear whether it is the bulk honey or some fraction of it. The antibacterial properties of honey speed up the growth of new tissue to heal the wound. The bactericidal effect of honey is reported to be dependent on concentration of honey used and the nature of the bacteria Considering the fact that their might be some specific constituents which may be contributing to the antimicrobial behaviour, it was decided to carryout the studies u sing different solvents.The present study therefore deals with the constituents in the different solvents followed by evaluation of extract for their antimicrobial behaviour against certain species of bacteria. 2) OBJECTIVE Based on the above facts, the present study was defined with following objective: 1) To check the antibacterial effect of different honeys on. a. Gram-positive Staphylococcus aureus, b. Gram-negative Escherichia coli, c. Gram-positive Bacillus subtilis, and d. Gram- negative Burkholderia capicium 2) To check the effect of honey on bacterial strains using different-different dilutions. . 100% honey sample b. 75% honey sample c. 50% honey sample d. 25% honey sample 3) To compare the effect of honey and control (Distilled water) on a. Gram-positive Staphylococcus aureus, b. Gram-negative Escherichia coli, c. Gram-positive Bacillus subtilis, and d. Gram- negative Burkholderia capicium 3) LITERATURE REVIEWS Honey has been used with medicinal purposes since ancient tim es and there are reports of its topic use by Ayurvedic medicine, dating back to 2500 b. c. Hippocrates prescribed the use of honey for several indications, including treatment of wounds and gastritis.Its healing properties are mentioned in the Koran and in Bible. Honey is a part of traditional medicine for centuries. The antimicrobial activity of honey is due primarily to hydrogen peroxide produced enzymatically. However, in undiluted honey the acidity is also a significant antimicrobial factor. The pH, ranging from 3. 2 to 4. 5, is low enough to inhibit the development of pathogens, whose optimal pH range situates between 7. 2 and 7. 4. When applied to affected areas of tissue, acidity, osmotic effect and phytochemical factors decrease with dilution; however, the activity of hydrogen peroxide increases from 2. to 50 times. On these values, peroxide acts as an antiseptic, without causing cell damage. The composition of sugars in honeys, from different floral origins, inhibits the de velopment of various intestinal bacteria. All chemical and physical factors make the therapeutic properties of honey unique: fast decline of infections and healing of wounds, quick inflammation recovery, minimizing of wounds, stimulation of angiogenesis, as well as the development of epithelial and granular tissues. Honey to be used with topical medicinal purpose, certain requirements are needed, like being free f herbicides, pesticides, heavy metals and radioactive elements and sterilized to prevent secondary infections. 3. 1) TYPES OF HONEY – ON BASIS OF FLORAL SOURCE- Blended- Most commercially available honey is blended, meaning it is a mixture of two or more honeys differing in floral source, color, flavor, density or geographic origin. Polyfloral – Polyfloral honey, also known as wildflower honey, is derived from the nectar of many types of flowers. The taste may vary from year to year, and the aroma and the flavor can be more or less intense, depending on which bloomings are prevalent.Monofloral- Monofloral honey is made primarily from the nectar of one type of flower. Different monofloral honeys have a distinctive flavor and color because of differences between their principal nectar sources. To produce monofloral honey, beekeepers keep beehives in an area where the bees have access to only one type of flower. Honeydew honey- Instead of taking nectar, bees can take honeydew, the sweet secretions of aphids or other plant sap-sucking insects. Honeydew honey is very dark brown in color, with a rich fragrance of stewed fruit or fig jam, and is not sweet like nectar honeys. . 2) COMPOSITION OF HONEY Carbohydrates Unsurprisingly, these comprise the major portion of honey – about 82%. The carbohydrates present are the monosaccharide fructose (38. 2%) and glucose (31%); and disaccharides (~9%) sucrose, maltose, isomaltose, maltulose, turanose and kojibiose. There are also some oligosaccharides present (4. 2%), including erlose, theanderose and panose, formed from incomplete breakdown of the higher saccharides present in nectar and honeydew. Proteins and Amino Acids.Honey contains a number of enzymes, including invertase, which converts sucrose to glucose and fructose; amylase, which breaks starch down into smaller units; glucose oxidase, which converts glucose to gluconolactone, which in turn yields gluconic acid and hydrogen peroxide; catalase, which breaks down the peroxide formed by glucose oxidase to water and oxygen; and acid phosphorylase, which removes inorganic phosphate from organic phosphates. Honey also contains eighteen free amino acids, of which the most abundant is proline. Vitamins, Minerals and AntioxidantsHoney contains trace amounts of the B vitamins riboflavin, niacin, folic acid, pantothenic acid and vitamin B6. It also contains ascorbic acid (vitamin C), and the minerals calcium, iron, zinc, potassium, phosphorous, magnesium, selenium, chromium and manganese. The main group of antioxidants in hon ey are the flavonoids, of which one, pinocembrin, is unique to honey and bee propolis. Ascorbic acid, catalase and selenium are also antioxidants. Generally speaking, the darker the honey, the greater its antioxidising properties. Other compoundsHoney also contains organic acids such as acetic, butanoic, formic, citric, succinic, lactic, malic, pyroglutamic and gluconic acids, and a number of aromatic acids. The main acid present is gluconic acid, formed in the breakdown of glucose by glucose oxidase. Honey also contains hydroxymethylfurfural, a natural product of the breakdown of simple sugars below pH 5. According to studies done by Patricia E. Lusby et al Twelve of the 13 bacteria were inhibited by all honeys used in this study with only Serratia marcescens and the yeast Candida albicans not inhibited by the honeys.Little or no antibacterial activity was seen at honey concentrations 1%, with minimal inhibition at 5%. No honey was able to produce complete inhibition of bacterial g rowth. Although Medi honey and manuka had the overall best activity, the locally produced honeys had equivalent inhibitory activity for some, but not all, bacteria. He then postulated that honeys other than those commercially available as antibacterial honeys can have equivalent antibacterial activity. These newly identified antibacterial honeys may prove to be a valuable source of future therapeutic honeys. According to Peter C.Molan (2001), honey may be the â€Å"natural cure† for most bacterial infections that could replace man-made antibiotics. Researchers say that an enzyme in the honey turns into a tiny  amount hydrogen peroxide when combined with bodily fluids killing nearby bacteria. Honey also causes an increase in lymphocyte and  phagocyte activity (Cooper et al. , 2011). The honeys are shown to have antibacterial properties, in particular Manuka honey. Manuka Honey has had extensive research done on it. It has been shown in many studies that Manuka Honey has an timicrobial effects (Barret J. , et. al, 2005; Coumbes A. L. , et. al. 2004; Mundo, MA. 2004).In this study the antibacterial activity of the five honeys were compared, the Manuka honey was used as a positive control. The nonperoxide antibiotic activity is due to methylglyoxal (MGO) and an unidentified synergistic component. Most honeys contain very low levels of MGO, but manuka honey contains very high levels. The presence of the synergist in manuka honey more than doubles MGO antibacterial activity. In vitro antibacterial activity of raw and commercially available honey was tested against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Shigella spp. nd Salmonella spp. ). Both types of honey showed antibacterial activity against test organisms with the zone of inhibition ranging from 8. 13 to 30. 85 mm, while E. coli, S. aureus, and Shigella spp. showed sensibility towards both types of Honey. Both types of honey showed no effects on Salmonella spp. The potency of honey at 100%concentration was found to be higher than all other concentrations tested. However, no effect was observed at concentration of 6. 25% v/v honey in the case of both samples(Patricia E. Lusby 2004).To study the antimicrobial activity of honey, 60 samples of various botanical origin were evaluated antimicrobial activities against 16 clinical pathogens and their respective reference strains. The bioassay applied for determining the antimicrobial effect employs the well-agar diffusion method and the estimation of minimum active dilution which produces a 1 mm diameter inhibition zone. Streptococcus pyogenes,Bacillus cereus and Bacillus subtilis were proven to be up to 60% more resistant than their equal reference strains thus emphasizing the variability in the antibacterial effect of honey and the need for further research (C.Voidarou 2010). Natural products, either as pure compounds or as standardized plant extracts, provid e unlimited opportunities for new drugs because of the ready availability of chemical diversity (Cos et al. , 2006). Honey and lemon-honey are traditional remedies in the Middle East and China and for many centuries and have been used in the treatment and prevention of the common cold and various upper respiratory tract infections (Molan, 1992; Zulma ; Lulat, 1989). Antibacterial activity of the honeys was assayed using standard well diffusion methods. oncluded that the sandal wood from different sources and mixture of of – and -santanols were highly effective against gram negative bacteria including Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae and as well as yeast Candida albicans. The present work gives strong evidence of antibacterial activities of honey, sandal oil and black pepper (Sheikh Ahmad 2002). In general, all types of honey have high sugar content as well as low water content and acidity, which prevent microbial growth.Osmotic effect, effect of pH and hydrogen peroxide are represented as an â€Å"inhibition† factor in honey (Postmes et al. , 1993). Most types of honey generate hydrogen peroxide when diluted because of the activation of the enzyme glucose oxidase, which oxidizes glucose to gluconic acid and hydrogen peroxide (Schepartz and Subers, 1964). Hydrogen peroxide is the major contributor to the antimicrobial activity of honey,and the different concentrations of this compound indifferent honeys result in their variable antimicrobial effects (Molan, 1992).Moreover, non peroxide factors also play critical role. The content of non peroxide factors are related to the floral source and sometimes account for the major part of the antibacterial activity in honey(Molan and Russell, 1988). Forty samples of different honey types (Acacia, Ziziphus , Brassica and Citrus) were collected from different areas of Pakistan and analyzed for moisture, pH, total acidity, ash, electrical conductivity, hydroxyl methyl furfural (HMF), sucrose, total sugars, invert sugar, protein, proline contents as well as macro and micro elements.The variation in composition of honey samples was observed due to different types of flora. Likewise, a significant level (P ; 0. 05) of ash, electric conductivity, sucrose, total sugar as well as macro and micro elements was also found in these honey types. Different formulations of honey has significantly inhibited growth of pathogenic microorganisms, Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger when compared to control group, which is an evidence that honey is a therapeutic agent being used since ancient time throughout the world. Feng et. al. 1994) The discrepancy in the observed antibacterial activity can be due to several reasons. One possibility might be related to the differences in susceptibility of each species of microorganism to the antibacterial activity of honey used. Similar observations are reported by others (Nzeako and Hamdi 2000; Ceyhan and Ugur 2001; Taormina et al. 2001). As reported by others (Melissa et al. 2004) dilution of honey enhances hydrogen peroxide mediated antibacterial activity may explain some of the discrepancies of observed with the antibacterial activity of these honey.The presence of unstable putative agents and/or thermolabile antibacterial agent(s) could also be inactivated during the experimental procedure and thus may be considered as possible explanation of the observed insensitivity of some honey samples found in the preliminary study. 4. ) MATERIALS AND METHOD 4. 1) Materials Several local brands of honey and Dabur honey collected from different sources is used in this study. Also the honey is collected from various apiaries from- Pathankot(Punjab) and Saharanpur (Up). In the antibacterial study, several bacterial species known to be pathogenic to human such as E. oli. , S. aureus and Burkholderia capicium and Bacillus subtilis was used. These strains were obtained from biote chnology lab. in accordance to given requirement. Glass wares used:- Conical flask (500 milliliter), Conical flask (100 milliliter), Petri plates, glass rod, test tubes spreader, glass beakers, Glass slides and cover slip, Plastic wares used:- Beakers, Conical flask (500 milliliter), Conical flask (100 milliliter), dropper, appendrof tubes (2ml), auto pipette tips (10? l, 100? l, and 1000? l) Instruments used:- Hot air oven, Autoclave machine, Laminar air flow hood, Incubator, Microwave ovenOther material used:- Sprit lamp, cork borer,test tube stand, auto pipette (10? l, 100? l, and 1000? l), parafilm wax,,filter paper Chemicals used:- Nutrient agar, nutrient broth, Mueller Hinton agar, 70% Methanol, 100% methanol, ethyl alcohol ,sodium chloride. 4. 2) Methods Physicochemical study: Appearance of different honeys was observed. pH is determined using conventional procedure like using ph scale. Antibacterial study: Antibacterial study was carried out in steps. In the first step, an i n vitro screening will be carried out using either disc diffusion or well diffusion method.Well diffusion was carried out using plate diffusion, which I preferred. Preparation of test materials: Test materials will be prepared by diluting each honey at different dilutions, 25 ? l/100 ? l, 50 ? l/100 ? l, 75 ? l/100 ? l and one with no dilution. Moreover, net honey was also used as test material. All dilutions were carried out with double distilled and deionised sterilized water. 3. 2. 1) Source of bacterial strains The E. coli. , S. aureus, Burkholderia capicium and Bacillus subtilis were revived from the stock available in the various Biotechnology laboratories of Lovely Professional University, Phagwara (Punjab), India.Sub-culturing of bacterial strains in nutrient broth:- 100ml nutrient broth was prepared and ten test tubes were taken, autoclaved, and after this the Bacterial strains was inoculated in the nutrient broth in different test tubes and then incubated for 24 hours in i ncubator . Sub-culturing of the bacterial strains on solid media:- For All Bacterial strains:- 5. 6g of Nutrient agar was added to the 200ml of purified water. Then solution was heated and boiled for 1 minute to completely dissolve the powder. Then media was autoclaved.Then media(10-15 ml) was poured in Petri plates. Then Bacterial strains were added to media. Then Petri plate were kept in incubator for 18-48 hours at 37 degree C. Saline preparation : Take . 58g of Nacl and dissolve it to 10ml of distled water and make saline before 15 min of spreading and take the colonies from the nutrient agar plates and mix properly in the saline. Nutrient agar medium for antimicrobial testing: 7g of nutrient agar medium was added to the 250 ml of distilled water and heated for 1 minute to complete dissolve medium then autoclaved .After that pouring the petriplates and left it 20 minute for solidify medium and make wells , after solidify put the different Honeys in the wells and kept it to incub ate for 18-48 hours at 37C. Testing of antibacterial activity using agar well diffusion method: The bacterial strains were inoculated into 10 ml of sterile nutrient broth, and incubated at 37  °C for 18 h. Each culture was then spreaded on the surface of sterile nutrient agar plate and also pour-plated in nutrient agar media to perform the test in triplet, one with the control and the other two ith the test sample. Making the wells With the help of a cork borer make wells in the agar carefully without tearing the gel. In two agar plate of all four sets, four wells were prepared with the help of sterilized cork borer. Then with the hep of micropipette put 100 micro litre of honey sample into the wellIn the wells of two plates of each set, samples of following concentrations: (1) 100% sterile Honey(2) 75% sterile Honey(3) 50% sterile Honey and (4) 25% sterile Honey; were added by using micropipette. Also in the a well water was added as negative control.Then with the help of micropi pette put 100 micro litre of honey sample into the well Replace the lid of the plate between putting sample into wells to minimize exposure to air-borne contaminants. Cover the lid tightly with paraffin tape to avoid contamination. Incubation of the plates . A temperature range of 35 °C  ± 2 °C is required for 24-48 hours. Do not incubate plates in carbon dioxide  as this will decrease the pH of the agar and result in errors due to incorrect pH of the media. Measuring zone of inhibition 1.Following incubation, measure the zone sizes  to the nearest millimeter  using a ruler or caliper; include the diameter of the well in the measurement . 2. All measurements were made with the unaided eye while viewing the back of the petri dish. Hold the plate a few inches above a black, nonreflecting surface illuminated with reflected light . 3. Record the zone size on the recording sheet. Figure1: Testing of antibacterial activity using agar well diffusion method 4. )RESULTS Sub-cultu ring of bacterial strains in nutrient broth:- Figure2: Culture E. coli. , S. ureus and Burkholderia capicium and Bacillus subtilis in Nutrient broth media. Sub-culturing of the bacterial strains on solid media:- Fig. –3 E. coli subcultured on nutrient agar Fig. – 4 B. Subtilis subcultured on nutrient agar Testing of antibacterial activity using agar well diffusion method: Fig 5 : No antibacterial activity seen as no honey is poured (taken as negative control) in which E. coli is grown Fig 6 : No antibacterial activity seen as no honey is poured (taken as negative control) in which Bacillus subtilis is grown ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST E. coliFIG. 7 – ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY(pathankot sample) IN E. coli: 100% = 2. 6cm, 75%= 1. 9cm, 50%= 1. 7cm,and 25%= 1. 2cm FIG. 8– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY (dabur sample) IN E. coli : 100% = 2. 2cm, 75%= 1. 6cm, 50%= 1. 3cm, and 25%= 1. 1cm FI G. 9– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY (saharanpur sample) IN E. coli: 100% = 2. 8cm, 75%= 2. 4cm, 50%= 2. 2cm, and 25%= 1. 7cm; ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST B. subtilis FIG. 10– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY IN B. subtilis (pathankot sample): 100% = 3. cm, 75%= 2. 7cm, 50%= 2. 7cm, and 25%= 2. 5cm FIG. 11– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY in B. subtilis (dabur sample): 100% = 2. 7cm, 75%= 2. 5cm, 50%= 2. 4cm, and 25%= 2. 2cm; FIG. 12– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY in B. subtilis (saharanpur):100% = 3. 2cm, 75%= 2. 7cm, 50%= 2. 3cm, and 25%= 1. 5cm ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST Burkholderia capacium FIG. 13– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY in Burkholderia capacium (pathankot sample): 100% = 2. 1cm, 75%= 1. 9cm, 50%= 1. 6cm, and 25%= 1. 4cm; FIG. 4– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY Burkholderia capacium (dabur): 100% = 2. 4cm, 75%= 1. 7cm, 50%= 1. 5cm, and 25%=1. 2cm FIG. 15– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY Burkholderia capacium (saharanpur):100% = 2. 5cm, 75%= 1. 9cm, 50%= 1. 7cm, and 25%=1. 4cm; ANTIMICROBIAL ACTIVITY OF VARIOUS HONEY’S AGAINST S. Aureus FIG. 16– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY S. aureus (pathankot):100%=2. 1cm, 75%= 1. 7cm, 50%= 1. 4cm, and 25%=1. 2cm FIG. 17– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY S. aureus (dabur):100% =3. 0cm, 75%= 2. 7cm, 50%= 2. 2cm, and 25%=1. cm FIG. 18– ZONES OF INHIBITION BY VARIOUS DILUTIONS OF HONEY IN S. aureus (saharanpur): 100% = 2. 9cm, 75%= 2. 5cm, 50%= 2. 0cm, and 25%=1. 6cm Appearance Appearance of each of honey brand was examined and it was observed that honey were brown to dark brown in color, whereas other honey were golden yellow in color. Dabur honey- golden colour,transparent Pathankot sample – golden colour wit h sufficient transparency Saharanpur sample – dark yellow colour with zero transparency The transparency is due to processing of honey,while less transparent samples are unprocessed and taken directly from bee hive.Table 1- Diameter of the Zone of Inhibition by different concentration of Pathankot honey sample on different Bacterial strains. Bacterial strains| Zone of Inhibition (in cm)| | Control(sterile DistilledWater)| 100%Honey sample| 75%Honey sample| 50%Honey sample| 25%Honey sample| E. coli. | 0. 0| 2. 6| 1. 9| 1. 7| 1. 2| Bacillus subtilis| 0. 0| 3. 0| 2. 7| 2. 7| 2. 5| S. aureus| 0. 0| 2. 1| 1. 7| 1. 4| 1. 2| Burkholderia capicium| 0. 0| 2. 1| 1. 9| 1. 5| 1. 4| Table2- Diameter of the Zone of Inhibition by different concentration of dabur honey sample on different Bacterial strains.Bacterial strains| Zone of Inhibition (in cm) | | Control(sterile DistilledWater)| 100%Honey sample| 75%Honey sample| 50%Honey sample| 25%Honey sample| E. coli. | 0. 0| 2. 2| 1. 6| 1. 3| 1 . 1| Bacillus subtilis| 0. 0| 2. 7| 2. 5| 2. 4| 2. 2| S. aureus| 0. 0| 3. 0| 2. 7| 2. 2| 1. 8| Burkholderia capicium| 0. 0| 2. 4| 1. 7| 1. 5| 1. 2| Table 1- Diameter of the Zone of Inhibition by different concentration of Saharanpur honey sample on different Bacterial strains. Bacterial strains| Zone of Inhibition (in cm)| | Control(sterile DistilledWater)| 100%Honey sample| 75%Honey sample| 50%Honey sample| 25%Honey sample| E. oli. | 0. 0| 2. 8| 2. 4| 2. 3| 1. 7| Bacillus subtilis| 0. 0| 3. 2| 2. 7| 2. 3| 1. 5| S. aureus| 0. 0| 2. 9| 2. 5| 2. 0| 1. 6| Burkholderia capicium| 0. 0| 2. 5| 1. 9| 1. 7| 1. 4| In the preliminary screening process was observed that some honey brands have more while some other have less antibacterial activity. Some honey showed antibacterial effect against E. Coli and some showed strongest activity against Bacillus subtilis ,S. aureus and Burkholderia capicium. Clear zones of inhibition were produced by concentrated without dilution.Through the analysis of average values of the diameters of each honey, it is possible to infer a possible pattern in which commercial honeys, such as DABUR honey, have a greater average diameter while the homemade honeys from different regions, have a lower average diameter even at less concentrations, but the best antimicrobial activity was shown by Saharanpur honey sample with was collected directly from the apiary without any processing and it showed clear and large inhibition zones for all bacterial strains which were used for this test.Though after the dilutions were made, the low concentrated samples of Saharanpur honey resulted in rather poor antimicrobial activity. The wells in which water was loaded shoed absolutely no antimicrobial activity which was used as negative control. The results also showed that lower concentration of honey made through dilution shows less antimicrobial activity than concentrated ones. Thus honey are definitely effective against the bacterial strains at all concentration s. 6) REFERENCES Bibi S, Husain SZ, Malik RN (2008). Pollen analysis and heavy metals detection in honey samples from seven selected countries. Pak. J. Bot. 40(2): 507-516 * Cooper R. A. , Molan P. C. , Harding K. G. (1999) Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds, J. R. Soc. Med. 92, 283–285. * Cooper R. How does honey heal wounds? In: Munn P, Jones R,editors. Honey and Healing. UK: International Bee Research Association; 2001. * de Jong H. J. 1999) The Land of Corn and Honey:The keeping of stingless bees (meliponiculture) in the ethno-ecological environment of Yucatan (Mexico) and El Salvador, Utrecht UniversityPress, Netherlands, 423 p. * Kamal A, Raza A, Rashid N, Hammed TG, Lami M, Gureshin MA,Nasim K (2002). Comparative study of Honey collected from flora of Pakistan. On Line J. Biol. Sci. 23(9): 626-627. * Khalil MI, Shahjahan M, Absar N (2006). Glycemic Response and Lycemic Index of Bangladeshi Honey in Type 2 Diabe tic Patients. Malaysian. J. Pharm. Sci. 4(1): 13–19. * Lusby PE, Coombes AL, Wilkinson JM.Bactericidal activity of different honeys against pathogenic bacteria. Arch Med Res 2005; 36: 464-467 * Molan P. C. (1997) The antibacterial activity of honey. The nature of the antibacterial activity,Bee World 73, 5–28. * Mundo MA, Padilla-Zakour OI, Worobo RW. Growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. Int J Food Microbiol 2004; 97: 1-8. * Tumin N, Halim NA, Shahjahan M, Noor Izani NJ, Sattar MA,Khan AH, et al. Antibacterial activity of local Malaysian honey. Malaysian J Pharma Sci 2005; 3: 1-10.